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Reference Assembly
GCA_001008285.1Single-End Sequencing Data
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ChIP-seq Analysis: Single-End Read Processing
Description
Complete ChIP-seq analysis for single-end sequencing data. Processes raw FASTQ files through adapter removal (cutadapt), alignment to reference genome (Bowtie2), and quality filtering (MAPQ >= 30). Peak calling with MACS2 uses either a fixed extension parameter or built-in model to identify protein-DNA binding sites. Generates alignment files, peak calls, and quality metrics for downstream analysis.